Born and raised in Chesterfield, north east Derbyshire, England, Guest started in modelling after she saw an advertisement while on a catering course at her local college. She appeared as aPage 3 girl in The Sun and has also appeared in the magazines Loaded and FHM. She also appeared in the Playboy video Shagalicious British Babes.
Guest appeared in a wide range of British "top shelf" magazines, including, Escort, Mayfair, Men Only, Men's World, Razzle and Whitehouse. Since 2000 she has been a television host for the Men and Motors cable/satellite channel in the UK, operated by Granada Television.
Guest also appeared in an interactive erotic magazine for PC called Interactive Girls. She starred in an erotic PC game Jo Guest in the Milk Round, released by Interactive Girls Club in 1994.
Starting with the June 2002 issue, Guest had a monthly advice column in Front magazine. She had previously appeared in a weekly "agony babe" advice column in the Daily Star newspaper (from November 1998 to March 2000).
- Jo Guest's Private Parts (Nov 1998)
- Undressed with Guest (the second series was shown in Spring 1999)
- Jo Guest in Jamaica (Jan-Feb 2000)
- Jo Guest’s Capital Exposed (Nov 2002)
- The Steam Room : with Jo Guest (Oct 2004)
- Jo Guest UK Exposed (Nov 2004)
- British Babes Exposed included The Steam Room hosted by Jo Guest (Oct 2008)
Guest also appeared in the third series of the British TV show, I'm Famous and Frightened!
On 21 January 2008, she was a guest on the television show This Morning, where she discussed a mystery illness she had been suffering from for the past 14 months that had left her unable to work and that doctors had been unable to diagnose. On 10 April 2008 she returned as a guest on This Morning and confirmed that, as a direct result of help from viewers that had seen her previous appearance, she had been diagnosed with fibromyalgia.